Large scale analysis of peptide-HLA class I interactions

In general, this data has been generated using a quantitative biochemical peptide-HLA interaction assay (see PMID: 12135423). The HLA class I molecules have been selected to cover the known HLA supertypes (using PMID: 14963618). Predictions of peptide binding to HLA class I has been performed using various bioinformatics approaches including artificial neural networks (see PMID: 14617044 �and PMID: 12717023 peptides and using a combined algorithm integrating MHC class I binding, TAP transport efficiency, and proteasomal cleavage predictions (PMID: 15997466). In many cases, the most informative peptides have been selected using a Query-by-Committeee procedure (using PMID: 14617044 and PMID: 14629874).