Germacrane-Type Sesquiterpenoids with Antiproliferative Activities from Eupatorium chinense

Ten new germacrane-type sesquiterpenoids (1–10) were isolated from a whole plant extract of Eupatorium chinense. The structures were elucidated by analysis of their NMR and MS data as well as by comparison with literature values. The absolute configuration of eupachinsin A (1) was determined by single-crystal X-ray diffraction analysis. Compounds 3 and 4 exhibited cytotoxicity against a human breast cancer cell line (MDA-MB-231), with IC50 values of 0.8 and 3.4 μM, respectively. In addition, compounds 3–5 showed cytotoxicity against the human hepatocellular carcinoma cell line (HepG2), with IC50 values ranging from 3.6 to 7.6 μM.本研究从华泽兰（Eupatorium chinense）全草提取物中分离得到10个全新的吉马烷型倍半萜类化合物（1–10）。通过核磁共振波谱（Nuclear Magnetic Resonance, NMR）与质谱（Mass Spectrometry, MS）数据分析，并结合文献报道数据比对，解析了所有化合物的结构。其中，化合物1（eupachinsin A）的绝对构型通过单晶X射线衍射分析得以确定。化合物3与4对人乳腺癌细胞系MDA-MB-231展现出细胞毒性，半数抑制浓度（Half Maximal Inhibitory Concentration, IC50）分别为0.8 μM和3.4 μM。此外，化合物3–5对人肝癌细胞系HepG2亦具有细胞毒性，其IC50值范围为3.6 μM至7.6 μM。