Biodegradable membrane coating recruited beneficial microbiota and increased rhizosphere bacterial diversity

The long-term use of ordinary plastic mulch has problems such as difficult recycling, large residual amount and heavy pollution, and the use of degradable mulch can not only have the same effect as ordinary mulch application, but also reduce material pollution. The effects of degraded film cover on microbial diversity were significant. However, the response of the relevant microbial communities to degraded film cover in different ecological niches (eg. bulk soil, rhizosphere and rhizosphere) of sunflower roots was still poorly understood. In this study, the effects of different mulching treatments on bacterial and fungal diversity, community assembly process, key dominant species and yield of sunflower root in different ecological niches were analyzed. The test results showed that the degradation membrane treatment significantly changed soil physical properties and increased soil enzyme activity, especially on AKP. At the same time, the degradation membrane treatment significantly reduced the diversity of rhizosphere and rhizosphere and increased the diversity of fungi, indicating that the degradation membrane treatment significantly affected the changes of rhizosphere and root endophyte. Film coating treatment can reduce the interference of non-film coating on the root-related niche bacterial and fungal communities, promote the transformation of microbial assembly to a deterministic process, promote the degradation film treatment and rhizosphere interaction, and increase the network interaction between beneficial microorganisms. Through the comprehensive analysis of random forest and structural equation, it is found that, The diversity of rhizosphere fungi became the main driving factor to increase the yield, which significantly increased the yield of sunflower. The yield of degraded black film (SH) in Shanghai was the highest, and that of control without film coating (CK) was the lowest. To provide theoretical support for stable yield increase and sustainable agricultural development of sunflower in this region.