Effect of coverage and sequencing error rates on θπ.

Average relative mean absolute deviation between the observed and expected value of θπ. Expectations were obtained from ms (sample_stats) and compared to the observed value calculated with PoPoolation for three different coverage values and three different sequencing error rates. The observed θπ was calculated assuming three different values of the minimum frequency of the alternative allele in the sequenced pool. Cov: Coverage.θπ观测值与预期值间的相对平均绝对偏差的平均值。 预期值通过ms软件的sample_stats模块获取，并与采用PoPoolation计算得到的观测值进行比对，本次比对涵盖三种不同测序覆盖度与三种不同测序错误率。 本次计算得到的观测θπ，基于测序混合样本中替代等位基因最小频率的三种不同取值条件获得。 Cov：测序覆盖度。