Global transcriptional profiles of primary and secondary cellular senescence in vivo by oncogene-induced senescence (OIS) and stress-induced senescence (SIS) [scRNA-seq]

The physiological roles of cellular senescence in vivo are largely unknown. We report primary and secondary senescence-induced transcriptional alteration by oncogene-induced senescence (OIS, ERK activation) and stress-induced senescence (SIS, p38 activation) of mouse hepatocytes. Our analyses revealed that each senescence-induced cell displays different transcriptional profiles explaining various known senescent properties. Hepatocytes were isolated from double-coloured chimeric control, caMEK1 (OIS), and caMKK6 (SIS) mice. After mCherry-positive primary senescent cells and GFP-positive surrounding cells were collected by using FACS, the sorted cells were subjected to scRNAseq analyses.