Complete-coding sequence of Ixeridium yellow mottle virus 2 isolated from Crepidiastrum sonchifolium in South Korea

The nearly complete genome sequence of Ixeridium yellow mottle virus 2 (IxYMV-2) was validated using partially overlapping RT-PCR fragments amplified with four primer sets, Sanger sequencing, and a 5’ RACE kit (v.20; Invitrogen). Attempts to perform 3’ RACE were unsuccessful. The consensus genome sequence (4404 nt, 43.31% GC content) was assembled using CLC Main Workbench v6.9.1 (Qiagen, Germany). This sequence includes the coding-complete region (3665 nt) along with the 5’ and 3’ untranslated regions.This dataset includes the raw Sanger sequencing data generated through one-step RT-PCR. The archive includes:.ab1 Files: Raw Chromatogram Trace Data from Capillary Electrophoresis..txt files: Base-called sequences are exported from chromatogram analysis software, available in either FASTA or plain text format.High-throughput sequencing (HTS) reads generated in parallel are available under BioProject SRR34505500. The assembled sequence has been deposited in GenBank under accession number PV911203.This dataset facilitates the molecular characterization of IxYMV-2 and is provided to ensure transparency and enable independent verification.苦荬菜黄斑病毒2（Ixeridium yellow mottle virus 2, IxYMV-2）的近乎完整基因组序列，通过四对引物扩增得到的部分重叠逆转录聚合酶链式反应（Reverse Transcription Polymerase Chain Reaction, RT-PCR）扩增片段、桑格测序（Sanger sequencing）以及5'末端快速扩增（5' RACE）试剂盒（v.20；Invitrogen）完成验证。针对3'末端快速扩增（3' RACE）的实验尝试未获成功。 共有基因组序列全长4404 nt，GC含量为43.31%，使用CLC Main Workbench v6.9.1（德国凯杰（Qiagen）公司）组装得到。该序列涵盖编码完整区域（3665 nt）以及5'和3'非翻译区。 本数据集包含通过一步法RT-PCR获得的原始桑格测序数据。存档文件包含：.ab1格式文件：毛细管电泳原始色谱轨迹数据；.txt格式文件：从色谱分析软件导出的碱基识别序列，可采用FASTA格式或纯文本格式。 同步生成的高通量测序（High-throughput Sequencing, HTS）读段可在BioProject数据库SRR34505500条目下获取。组装得到的序列已提交至基因银行（GenBank），登录号为PV911203。 本数据集可为IxYMV-2的分子特征解析提供支撑，且公开该数据集以保障研究透明度并支持独立验证。