Data from: SNP markers tightly linked to root knot nematode resistance in grapevine (Vitis cinerea) identified by a genotyping-by-sequencing approach followed by Sequenom MassARRAY validation

Plant parasitic nematodes, including root knot nematode Meloidogyne species, cause extensive damage to agriculture and horticultural crops. As Vitis vinifera cultivars are susceptible to root knot nematode parasitism, rootstocks resistant to these soils pests provide a sustainable approach to maintain grapevine production. Currently, most of the commercially available root knot nematode resistant rootstocks are highly vigorous and take up excess potassium, which reduces wine quality. As a result, there is a pressing need to breed new root knot nematode resistant rootstocks, which have no impact on wine quality. To develop molecular markers that predict root knot nematode resistance for marker assisted breeding, a genetic approach was employed to identify a root knot nematode resistance locus in grapevine. To this end, a Meloidogyne javanica resistant Vitis cinerea accession was crossed to a susceptible Vitis vinifera cultivar Riesling and results from screening the F1 individuals support a model that root knot nematode resistance, is conferred by a single dominant allele, referred as MELOIDOGYNE JAVANICA RESISTANCE1 (MJR1). Further, MJR1 resistance appears to be mediated by a hypersensitive response that occurs in the root apical meristem. Single nucleotide polymorphisms (SNPs) were identified using genotyping-by-sequencing and results from association and genetic mapping identified the MJR1 locus, which is located on chromosome 18 in the Vitis cinerea accession. Validation of the SNPs linked to the MJR1 locus using a Sequenom MassARRAY platform found that only 50% could be validated. The validated SNPs that flank and co-segregate with the MJR1 locus can be used for marker-assisted selection for Meloidogyne javanica resistance in grapevine.

植物寄生线虫，包括根结线虫属（Meloidogyne）物种，对农业及园艺作物造成大范围危害。欧亚种葡萄（Vitis vinifera）品种易受根结线虫寄生，因此对这类土传害虫具有抗性的砧木可为维持葡萄生产提供可持续路径。目前，市面上多数具备根结线虫抗性的砧木生长势过强，会过量吸收钾元素，进而降低葡萄酒品质。因此，亟需培育出既抗根结线虫又不会影响葡萄酒品质的新型葡萄砧木。为开发可用于标记辅助育种的根结线虫抗性预测分子标记，本研究采用遗传学方法在葡萄中鉴定出一个根结线虫抗性位点。为此，本研究将抗爪哇根结线虫（Meloidogyne javanica）的河岸葡萄（Vitis cinerea）种质材料与易感根结线虫的欧亚种葡萄品种雷司令（Riesling）进行杂交，对F1个体的筛选结果支持“根结线虫抗性由单个显性等位基因介导”的模型，该等位基因被命名为爪哇根结线虫抗性1（MELOIDOGYNE JAVANICA RESISTANCE1，MJR1）。进一步研究表明，MJR1介导的抗性通过发生在根顶端分生组织的超敏反应实现。研究人员通过测序分型技术鉴定出单核苷酸多态性（SNPs），关联分析与遗传定位结果显示，MJR1位点位于该河岸葡萄种质材料的18号染色体上。采用Sequenom MassARRAY平台对与MJR1位点连锁的SNPs进行验证，结果显示仅有50%的SNPs可被成功验证。与MJR1位点侧翼区域共分离的经验证SNPs，可用于葡萄抗爪哇根结线虫的标记辅助选择育种。