LncRNA and mRNA profiling of the ischemia zone, the border zone and the remote non-ischemia zone of murine myocardial ischemia/reperfusion (MI/R) hearts

Purpose：Detection of differentially expressed lncRNA in the infarct zone and the control group in myocardial ischemia-reperfusion injury model tissue. Method: Use 8 weeks of C57BL/6 mice to establish a myocardial ischemia-reperfusion injury model, 45 minutes of ischemia, and 24 hours after reperfusion, the mice were sacrificed to obtain materials. Result: The expression of lncRNAs in the infarct area of myocardial ischemia-reperfusion injury model mice was detected, and it was found that a total of 43 lncRNAs related to myocardial ischemia-reperfusion injury changed in expression, of which 17 were up-regulated (fold change >1.5). 26 expressions are down-regulated (fold change <0.8) Three C57BL/6 mice (8 weeks old) were subjected to the 45 mins ligation of left anterior descending coronary artery followed 24h reperfusion. Then The heart were removed after the mice were euthanized and the left ventricle tissue were separated into normal zone, ischemia zone and ischemia border zone. Total RNA was isolated with TRIzol (Invitrogen) from heart tissue according to manufacturer’s instructions.LncRNA and mRNA expression profiling was performed using Affymetrix GeneChip Mouse Transcriptome Assay 1.0 according to the manufacturer’s instructions (Affymetrix, Inc., Santa Clara, CA).