RMSD and fluorimeter data for "Structural transitions in kinesin minus-end directed microtubule motility"

This entry is for our manuscript, "Structural transitions in kinesin minus-end directed microtubule motility" by Satoki Shibata*, Matthew Y. Wang*, Tsuyoshi Imasaki*, Hideki Shigematsu, Yuanyuan Wei, Chacko Jobichen, Hajime Hagio, J. Sivaraman, Sharyn A. Endow & Ryo Nitta. *Equal contribution Corresponding Authors: Tsuyoshi Imasaki*, Sharyn A. Endow, Ryo Nitta The deposited datasets are 1) beta-strand residue all atom RMSD between kinesin-14 NcdY485K ADP and ADP + free Pi nucleotide states and 2) fluorimeter assays of changes in intrinsic NcdY485K fluorescence upon addition of free Pi . The data are presented in Fig. 7e, and Fig. 8b,c and Sup Fig 2, respectively. Files are named for the figures in which the data are shown. A computer script for the RMSD analysis is included. Methods for the fluorimeter assays and data analysis are given.本条目对应我们的手稿《驱动蛋白负端定向微管运动的结构转变》，作者为Satoki Shibata*、Matthew Y. Wang*、Tsuyoshi Imasaki*、Hideki Shigematsu、Yuanyuan Wei、Chacko Jobichen、Hajime Hagio、J. Sivaraman、Sharyn A. Endow 与 Ryo Nitta。 * 共同第一作者 通讯作者：Tsuyoshi Imasaki*、Sharyn A. Endow、Ryo Nitta 本数据集包含两部分内容：1）驱动蛋白-14 NcdY485K的ADP状态与ADP+游离Pi核苷酸状态之间的β折叠残基全原子均方根偏差（RMSD）数据；2）添加游离Pi后NcdY485K固有荧光变化的荧光分光光度测定数据。上述数据分别对应图7e、图8b、c以及补充图2，数据集文件以其对应展示数据的图表命名。本数据集还包含用于RMSD分析的计算机脚本，荧光分光光度测定法及数据分析的相关方法已提供。