poly(A+) RNAs 3'-seq analysis in the cytoplasmic RNAs of control and U1 AMO treated Hela cells

To understand if U1-AMO induced truncated forms of mRNAs could be exported into cytoplasm, we performed 3'-seq analysis in the cytoplasmic RNAs of control and U1 AMO treated Hela cells Overall design: Control and U1 AMO were transfected into Hela cells, cytoplasmic fractions were isolated and RNAs were subsequently purified by Trizol reagent. 3'-seq analysis was performed using lexogen kit (016.024)