H460-RUNX3.xlsx

To identify genes regulated by RUNX3, we performed mRNA sequencing (RNA-seq) in H460-ERT2-RUNX3 and H460-ERT2-RUNX3-K94/171R cells. H460-vec, H460-ERT2-RUNX3, and H460-ERT2-RUNX3-K94/171R cells were serum-starved for 24 hr, and then stimulated with 10% serum or 10% serum + 1mM 4-OHT for 0, 8, or 16 hr. RNA was extracted from the cells, and gene expression patterns were analyzed by mRNA sequencing. Expression of genes 8 or 16 hr after serum stimulation was quantified as log₂(fold change) relative to the average of control reactions (i.e., before serum stimulation, 0 hr) for each cell line.为鉴定受RUNX3调控的基因，我们在H460-ERT2-RUNX3与H460-ERT2-RUNX3-K94/171R细胞中开展了mRNA测序（RNA-seq）。将H460-vec、H460-ERT2-RUNX3及H460-ERT2-RUNX3-K94/171R细胞进行24小时血清饥饿处理，随后分别用10%血清或10%血清+1mM 4-OHT刺激0、8或16小时。收集细胞并提取RNA，通过mRNA测序分析基因表达谱。以各细胞系血清刺激前（0小时）的对照样本平均值为基准，将血清刺激后8小时或16小时的基因表达量以log₂(倍数变化)进行定量。