Additional file 7: of A comparison of heat-stress transcriptome changes between wild-type Arabidopsis pollen and a heat-sensitive mutant harboring a knockout of cyclic nucleotide-gated cation channel 16 (cngc16)
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RNA-Seq validation using real-time Q-PCR. a. Comparison of expression values obtained from Q-PCR and RNA-Seq normalized to WT control (normal). The analysis was performed on two different reference genes separately (CYCP2 (AT3G21870) and UBQ7 (AT2G35635)). b. Primer sequences used for real-time Q-PCR. (XLSX 16 kb)
基于实时定量聚合酶链式反应（real-time Q-PCR）的RNA测序（RNA-Seq）验证实验。 a. 对比实时Q-PCR与RNA-Seq所得的、以野生型对照（正常组）为参照进行标准化的表达量值。本分析分别针对两个不同的内参基因（CYCP2（AT3G21870）和UBQ7（AT2G35635））开展。 b. 实时Q-PCR所用引物序列。（XLSX 16 kb）
创建时间：
2018-07-25
