Expression of PtoxA in the wild-type, ptxS and ptxR deficient backgrounds.
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https://figshare.com/articles/dataset/_Expression_of_P_toxA_in_the_wild_type_ptxS_and_ptxR_deficient_backgrounds_/275922
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The promoter region of toxA or kgu was cloned into pMP220 (TcR) and the resulting plasmid electroporated into the indicated strains. Cells were grown on M9 minimal medium with citrate (15 mM) and overnight cultures were diluted 50-fold in the same medium in the absence or in the presence of 2-ketogluconate (5 mM). β-galactosidase activity was determined in cells in the exponential phase of growth after 3 hours of incubation. Data are the average of 3 independent assays each performed in duplicate.
创建时间：
2015-12-02
