Tabula Sapiens v2

Tabula Sapiens is a benchmark, first-draft human cell atlas of over 1.1M cells from 28 organs of 24 normal human subjects. This work is the product of the Tabula Sapiens Consortium. Taking the organs from the same individual controls for genetic background, age, environment, and epigenetic effects, and allows detailed analysis and comparison of cell types that are shared between tissues. Our work creates a detailed portrait of cell types as well as their distribution and variation in gene expression across tissues and within the endothelial, epithelial, stromal and immune compartments.We have built directly on our unique skills, experience, and data infrastructure from Tabula Muris and Tabula Muris Senis to create a high-quality human reference dataset and portal at a 10-fold larger scale from these prior efforts.A critical factor in the Tabula projects is our large collaborative network of PI’s with deep expertise at preparation of diverse organs, enabling all organs from a subject to be successfully processed within a single day. We have built the logistics and infrastructure capable of tracking hundreds of samples and thousands of 384-well plates from tissue through sample prep, library construction and on to sequencing and ultimately computational and expert cell annotation with tight quality control.Tabula Sapiens leverages our network of human tissue experts and a close collaboration with a Donor Network West, a not-for-profit organ procurement organization. We use their experience to balance and assign cell types from each tissue compartment and optimally mix high-quality plate-seq data and high-volume droplet-based data to provide a broad and deep benchmark atlas.With the second version, Tabula Sapiens 2.0, we have built an integrated map of 28 tissues collected across 24 donors. Nine new donors and four new tissues were collected and analyzed together with the original Tabula Sapiens 1.0 dataset. All tissues and organs, with the exception of the respective reproductive organs, were profiled from both male (n=11) and female (n=13) donors. The donor's age ranges from 22 to 74 years old, offering one of the most comprehensive molecular profiles of human tissues across the adult lifespan (7 donors under the age of 40, 11 donors between 40 and 60, and 6 donors over 60 years of age).

Tabula Sapiens是首个人类细胞图谱基准草案，包含来自24名健康人类受试者28个器官的超110万个细胞。该成果由Tabula Sapiens联盟完成。通过采用同一受试者的器官样本，可有效控制遗传背景、年龄、环境及表观遗传带来的混杂效应，进而可对不同组织中共有的细胞类型开展精细分析与比较。本研究详细刻画了各类细胞类型的特征，以及它们在不同组织间、在内皮、上皮、基质与免疫细胞区室中的分布模式与基因表达差异。本研究依托此前在小鼠细胞图谱（Tabula Muris）与衰老小鼠细胞图谱（Tabula Muris Senis）项目中积累的独特技术、实践经验与数据基础设施，构建了规模较此前项目提升10倍的高质量人类参考数据集与数据门户。Tabula系列项目的核心优势之一，是我们拥有由多位具备多样器官样本制备资深经验的首席研究员（Principal Investigator，PI）组成的大型协作网络，可实现单一名受试者的全部器官在单日之内完成标准化处理。我们搭建了完备的物流与信息管理基础设施，可实现从组织样本制备、文库构建、高通量测序，直至最终计算分析与专家细胞注释的全流程严格质量管控，并可追踪数百份样本与数千块384孔板的完整实验链路。Tabula Sapiens项目依托我们的人类组织专家团队，并与非营利性器官获取机构Donor Network West开展紧密合作。我们借助其专业经验，对各组织区室的细胞类型进行校准与注释，并优化整合高质量平板测序（plate-seq）数据与高通量液滴测序数据，从而构建出覆盖全面、解析度优异的基准图谱。在第二版Tabula Sapiens 2.0中，我们构建了覆盖24名供体的28种组织的整合图谱。本次新增了9名供体与4种组织的样本，并与原始的Tabula Sapiens 1.0数据集合并分析。除各供体对应的生殖器官外，其余所有组织与器官均采集自男性（n=11）与女性（n=13）供体。供体年龄跨度为22至74岁，涵盖了成年生命周期各阶段的人类组织分子谱（其中7名供体年龄小于40岁，11名介于40至60岁，6名大于60岁）。

搜集汇总

背景与挑战

背景概述

Tabula Sapiens v2是一个大规模的人类单细胞转录组学数据集，包含超过110万个细胞，来自24名正常受试者的28个器官，旨在构建一个基准性的人类细胞图谱。该数据集通过从同一受试者收集多个器官，控制了遗传背景、年龄和环境因素，便于跨组织细胞类型的详细分析和比较。其特点包括整合了早期版本数据，新增捐赠者和组织，覆盖22至74岁的年龄范围，并使用10X和smartseq技术进行高质量数据生成。