Leishmania_braziliensis_gp63_functional_domain_sequence 3.fas

DNA sequence of the gp63 fragment encoding the functional domain of leishmanolysin in natural isolates of Leishmania braziliensis from Corte de Pedra, Northeast Brazil: allele 3. The stretch of gp 63 previously described as encoding the functional domain of the molecule was amplified by PCR, cloned into pcr 2.1 vector and bidirectionally sequenced. Sequences from a panel of 41 parasite isolates were aligned and 45 alleles of the fragment could be identified based on indel and SNPs presented among cloned domains. Haplotypes of polymorphisms (indel and SNPs) that could be detected in at least two clones of the gp63 domain in a single isolate, and in at least two parasite isolates in the collection were considered alleles. Corte de Pedra is a region highly endemic for American tegumentary leishmaniasis (ATL) due to L. braziliensis. Several forms of ATL occur and close to 1,500 new cases are diagnosed in the region each year.<br><br>巴西东北部Corte de Pedra地区巴西利什曼原虫（Leishmania braziliensis）自然分离株中编码利什曼蛋白酶（leishmanolysin）功能域的gp63片段的DNA序列：等位基因3。此前被描述为编码该分子功能域的gp63区段经聚合酶链式反应（Polymerase Chain Reaction，PCR）扩增后，被克隆至pcr2.1载体并完成双向测序。研究对41株寄生虫分离株的序列进行比对后，基于克隆获得的功能域中存在的插入缺失多态性（insertion-deletion polymorphism，indel）与单核苷酸多态性（Single Nucleotide Polymorphism，SNP，复数形式SNPs），可鉴定出该片段共计45种等位基因。针对单株分离株中至少两个gp63功能域克隆，以及该菌株收集库中至少两株寄生虫分离株均可检测到的多态性（插入缺失与单核苷酸多态性）单倍型，被界定为等位基因。 Corte de Pedra地区是由巴西利什曼原虫引发的美洲皮肤利什曼病（American Tegumentary Leishmaniasis，ATL）高流行区域，当地存在多种临床类型的美洲皮肤利什曼病，每年新增确诊病例近1500例。