High-confidence predicted miRNA coding sequences on chromosome 1AL.

1Where two similar known miRNAs gave equally close matches to a sequence, the evolutionarily closest match is given.2Matched sequence reads shown in bold were also predicted to form miRNA hairpins by miRPara.3Mismatches to the conserved miRNA sequence are underlined and in bold.4MFE = Minimum Folding free Energy of predicted hairpin secondary structure.5MFEI = Minimum Folding Energy Index, calculated as described by Yin et al. [40].6miRPara did not predict these hairpins, but predicted a pre-miRNA on the complementary strand. For F003IAL01CL160, which contains two adjacent pre-miRNA hairpins, miRPara predicted the same strand for one but the complementary strand for the other.1 当两条相似的已知微小RNA（miRNAs）与某一序列的匹配度不相上下时，选取进化关系最为密切的匹配结果。 2 以粗体显示的匹配序列读段，同时被miRPara预测可形成微小RNA茎环结构。 3 与保守型微小RNA序列不匹配的碱基将被加下划线并以粗体显示。 4 MFE（Minimum Folding free Energy）：即预测所得茎环二级结构的最小折叠自由能。 5 MFEI（Minimum Folding Energy Index）：按照Yin等人[40]所述方法计算得到。 6 miRPara未预测到上述茎环结构，但在互补链上预测到了前体微小RNA（pre-miRNA）。针对包含两个相邻前体微小RNA茎环的F003IAL01CL160序列，miRPara对其中一个预测位于正义链，对另一个则预测位于互补链。