(high-temp) No 3. Filtering: (16S rRNA/ITS) Output

**Output files from the No 3. Filtering Workflow** page of the SWELTR high-
temp study. ASV filtering for 16S rRNA & ITS using **a**) Arbitrary filtering,
**b**) PERFect (PERmutation Filtering test for microbiome data), and **c**)
PIME (Prevalence Interval for Microbiome Evaluation) Workflow objects:

**filtering_wf.rdata:** contains all variables and phyloseq objects from 16s
rRNA and ITS ASV filtering. To see the **Objects** , in R run
_load("__filtering_wf.rdata", verbose=TRUE)_

Additional files:

For convenience, we also include individual phyloseq objects for each filtered
data set.

**_Arbitrary_ :
**

****ssu18_ps_filt.rds:**** phyloseq object for Arbitrary filtered 16S rRNA
ASVs.****
its18_ps_filt.rds:**** phyloseq object for Arbitrary filtered ITS ASVs.****

****

**_PERfect_ :**

******ssu18_ps_perfect.rds** : ****phyloseq object for PERfect filtered 16S
rRNA ASVs.****
**its18_ps_perfect.rds** : ****phyloseq object for PERfect filtered ITS
ASVs.****
****

****_PIME_ : ** **

**ssu18_ps_pime.rds** : phyloseq object for PIME filtered 16S rRNA ASVs.
**its18_ps_pime.rds** : phyloseq object for PIME filtered ITS ASVs.

**Source code** for the workflow can be found here:

https://github.com/sweltr/high-temp/blob/master/filtering.Rmd

**SWELTR高温研究第3号过滤工作流页面的输出文件**。针对16S rRNA与ITS序列，采用以下三种方式完成**扩增子序列变异（ASV，Amplicon Sequence Variant）**过滤：**a**）任意过滤法，**b**）PERFect（微生物组数据置换过滤测试，PERmutation Filtering test for microbiome data），**c**）PIME（微生物组评估患病率区间，Prevalence Interval for Microbiome Evaluation）工作流对象：

**`filtering_wf.rdata`**：包含16S rRNA与ITS的ASV过滤流程中生成的全部变量与phyloseq对象。若需查看其中包含的对象，可在R语言环境中执行代码`load("__filtering_wf.rdata", verbose=TRUE)`。

**附加文件**
为便于使用，本数据集还附带了各过滤后数据集对应的独立phyloseq对象：

**_任意过滤法组_：**
**`ssu18_ps_filt.rds`**：对应经任意过滤法处理的16S rRNA ASV的phyloseq对象。
**`its18_ps_filt.rds`**：对应经任意过滤法处理的ITS ASV的phyloseq对象。

**_PERFect过滤组_：**
**`ssu18_ps_perfect.rds`**：对应经PERFect过滤法处理的16S rRNA ASV的phyloseq对象。
**`its18_ps_perfect.rds`**：对应经PERFect过滤法处理的ITS ASV的phyloseq对象。

**_PIME过滤组_：**
**`ssu18_ps_pime.rds`**：对应经PIME过滤法处理的16S rRNA ASV的phyloseq对象。
**`its18_ps_pime.rds`**：对应经PIME过滤法处理的ITS ASV的phyloseq对象。

本工作流的源代码可通过以下链接获取：
https://github.com/sweltr/high-temp/blob/master/filtering.Rmd