Additional file 5: Figure S3. of The role of Tre6P and SnRK1 in maize early kernel development and events leading to stress-induced kernel abortion

Effect of sucrose starvation on in vitro cultured kernels (3 DAP). Kernels were excised from field grown maize plants 3 days after pollination. After sterilization, kernels were plated on MS agar and incubated in the dark at 24 °C. Control kernels on continuous sucrose (150 mM) are indicated with closed (black) squares and sucrose starved kernels are indicated by white (open) squares. The shaded area highlights the 48 h period of starvation, and the unshaded area indicates the period on recovery on sucrose. Means are calculated using 4 biological replicates consisting of 3 plants each, and significance as reported by the Student’s T-test is at α = 0.05. (XLSX 85 kb)蔗糖饥饿对授粉后3天（3 DAP，days after pollination）离体培养玉米籽粒的影响。实验材料取自大田种植的玉米植株授粉后3天切取的籽粒。经灭菌处理后，将籽粒接种于MS培养基（MS agar）琼脂平板上，于24℃黑暗条件下培养。持续添加150 mM蔗糖的对照组籽粒以实心（黑色）方块标记，蔗糖饥饿处理组籽粒以空心（白色）方块标记。阴影区域代表48小时的饥饿处理阶段，非阴影区域则为蔗糖恢复培养阶段。均值计算采用4次生物学重复，每次重复包含3株植株；显著性检验采用t检验（Student’s T-test），显著性水平α=0.05。(XLSX 85 kb)