CIL:21744

Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA, CIL 21789) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and mad2, CIL 21749, 21739, 21759, and 21773, respectively) has a different gene knocked down by RNAi. This is the set that were treated with 340 RNAi. There are 10 images of each sample, for a total of 50 images. The images were acquired on a Zeiss Axiovert 200M microscope. A recommended use for these five image sets is to algorithmically compute the number of cells in each image. A tab-delimited text file containing this information can be found at the URL indicated in the attribution box. The recommended citation when using these images is: We used the Drosophila Kc167 1 image set (Carpenter, et al., Genome Biology, 2006) available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc).本数据集包含5份经DNA染料赫斯特33342（Hoechst 33342）染色的黑腹果蝇（Drosophila melanogaster）Kc167细胞样本。其中最后一份样本标记为nodsRNA，编号CIL 21789，为野生型细胞样本；其余4份样本分别标记为48、340、Anillin、mad2，对应编号依次为CIL 21749、21739、21759、21773，每份样本均通过RNA干扰（RNAi）技术敲低了不同的靶基因，其中标记为340的样本属于经340 RNAi处理的组别。每份样本配有10张成像图，总计50张图像。所有图像均通过蔡司（Zeiss）Axiovert 200M显微镜采集。本数据集的推荐应用方向为通过算法自动计算单张图像中的细胞数量。包含对应统计信息的制表符分隔文本文件可在标注栏指定的网址获取。使用本图像集时的推荐引用格式为：我们使用了来自Broad生物图像基准集（Broad Bioimage Benchmark Collection，www.broad.mit.edu/bbbc）的黑腹果蝇Kc167图像集1（Carpenter等人，《基因组生物学》，2006年）。