Supplementary Material - Independent control over cell patterning and adhesion on hydrogel substrates for tissue interface mechanobiology

We describe a technique for photopatterning single-stranded DNA (ssDNA) oligonucleotides onto photoactive benzophenone-containing polyacrylamide hydrogels (BP-PA hydrogels) combined with extracellular matrix (ECM) protein functionalization of hydrogels. ssDNA features are used to capture lipid-ssDNA labeled cells, while ECM proteins support subsequent cell adhesion. This paper contains a method description for cell patterning, measurements of cell-hydrogel interaction, and mechanical and functional characterization of BP-PA hydrogel substrates. In the attached dataset, we have included our photomask designs, microindentation dataset, and analysis code. Supplementary data includes: -Photomask design files in GDS II format (.gds) -Matlab analysis scripts for microindentation measurements (.m) -Individual measurements of hydrogels with varying Am/Bis composition (.txt)