Wire-tailed Manakin Telomere Analysis (V2)

1.Building on the predictions of state-dependent life history theory, telomeres are hypothesized to either correlate with or function as an adaptive, proximate mediator of an individual’s behavior and life-history strategy. To further understand the relationship between telomeres, behavior, and life-history strategies, we measured male behavior, telomere lengths, and telomere dynamics in a free-living population of known-age, male wire-tailed manakins (Pipra filicauda). 2.Male wire-tailed manakins perform coordinated displays with other males at leks and these displays form the basis of long-term coalition partnerships. Males exhibit consistent individual differences in the number of social partners within their social network and the frequency of social interactions. Male sociality is also positively correlated with both social rise and reproductive success. 3.We measured male behavior using a telemetry-based, proximity datalogging system and blood telomere lengths were quantified using qPCR. We examined the relationships between telomere length, telomere dynamics, social status, and male behavior. We also quantified the repeatability of telomere lengths, examined age-related changes in telomere length, and tested for instances of telomere elongation that exceed residual error in telomere length. 4.Telomere length was found to be highly repeatable. More social males exhibited shorter telomeres and higher rates of telomere attrition. Telomeres did not significantly vary with age within or between individuals in either of the male social classes. Two out of 25 individuals exhibited patterns telomere elongation that exceeded residual error in telomere measurements. 5.Here we show that telomeres consistently vary between male wire-tailed manakins and these differences are related to variation in male social behavior. In this relatively long-lived species, telomeres also appear to be flexible traits that can increase or decrease in length. Overall, this study provides observational support for the hypothesis that telomeres act as a molecular marker that relates to behavior in a state-dependent manner. We also provide insight into the molecular consequences of individual variation in male social behavior. This dataset was updated on 20201109. The original dataset can be found at the following DOI, doi:10.7294/mpcg-qx451. 基于状态依赖生活史理论的预测，端粒（telomeres）被假设为与个体行为和生活史策略相关联，或是作为二者的适应性近端介导因子。为进一步阐明端粒、行为与生活史策略之间的关联，我们在一个已知年龄的野生雄性线尾侏儒鸟（Pipra filicauda）种群中，测定了雄性个体的行为、端粒长度与端粒动态变化。 2. 雄性线尾侏儒鸟会在求偶场（leks）与其他雄性协同进行求偶展示，这类展示构成了长期联盟伙伴关系的基础。雄性个体在社交网络中的社交伙伴数量及社交互动频率方面，存在稳定的个体差异。雄性社交性同时与社会地位晋升及繁殖成功率呈正相关。 3. 我们采用基于遥测的邻近数据记录系统对雄性行为进行测定，并通过定量聚合酶链式反应（qPCR）量化血液端粒长度。我们分析了端粒长度、端粒动态、社会地位与雄性行为间的关联。同时，我们量化了端粒长度的重复性，考察了端粒长度随年龄的变化趋势，并检验了端粒长度超出测量残余误差的伸长案例。 4. 研究发现端粒长度具有高度的重复性。社交性更强的雄性个体端粒更短，端粒磨损速率也更高。在两类雄性社会阶层中，端粒长度并未随个体内部或个体之间的年龄发生显著变化。25个受试个体中有2个呈现出端粒伸长超出测量残余误差的模式。 5. 本研究表明，雄性线尾侏儒鸟的端粒长度存在显著个体差异，且这些差异与雄性社交行为的变异密切相关。在这一相对长寿的物种中，端粒似乎也是一种可塑性状，其长度可出现增加或减少的变化。总体而言，本研究为"端粒作为以状态依赖方式关联行为的分子标记"这一假说提供了观测支持证据。同时，我们也为雄性社交行为的个体变异所带来的分子层面后果提供了新的研究见解。本数据集于2020年11月9日完成更新。原始数据集可通过以下DOI获取：doi:10.7294/mpcg-qx45