Action of enfuvirtide on the pregnancy of albino rats ( Rattus norvegicus albinus, Rodentia, Mammalia ): biological assay and functional and histological analyses of exposed maternal-fetal organs

ABSTRACT Objective To assess the effects of enfuvirtide on pregnancy in albino rats and their fetuses. Methods Forty pregnant EPM 1 Wistar rats were randomly allocated into four groups: control (E) (distilled water twice/day), G1 (4mg/kg/day enfuvirtide), G2 (12mg/kg/day enfuvirtide), and G3 (36mg/kg/day enfuvirtide) groups. On the 20th day of gestation, the rats were anesthetized and subjected to cesarean section. Their blood was collected for laboratory analysis, and they were sacrificed. The offspring’s fragments of their kidneys, liver, and placentas and the maternal rats’ fragments of their lungs, kidneys, and liver were separated in the immediate postpartum period for light microscopy analysis. Results No maternal deaths occurred. In the second week at the end of pregnancy, the mean weight of the G3 Group was significantly lower than that of the G2 Group (p=0.029 and p=0.028, respectively). Analyzing blood laboratory parameters, the G1 Group had the lowest mean amylase level, and the G2 Group had the lowest mean hemoglobin level and the highest mean platelet count. In the morphological analysis, there were no changes in organs, such as the kidneys and liver, in both the maternal rats and offspring. Three maternal rats in the G3 Group had pulmonary inflammation in the lungs. Conclusion Enfuvirtide has no significant adverse effects on pregnancy, conceptual products, or functional alterations in maternal rats.摘要 目的 评估恩夫韦肽（enfuvirtide）对白化大鼠及其胎仔妊娠结局的影响。方法 将40只孕EPM 1品系Wistar大鼠随机分为4组：对照组（E组，每日两次给予蒸馏水）、G1组（4mg/kg/d恩夫韦肽）、G2组（12mg/kg/d恩夫韦肽）及G3组（36mg/kg/d恩夫韦肽）。于妊娠第20日，对大鼠实施麻醉并剖宫产，采集血液进行实验室检测后处死大鼠。产后即刻分离子代大鼠的肾脏、肝脏、胎盘组织片段以及母鼠的肺脏、肾脏、肝脏组织片段，用于光学显微镜分析。结果 本研究无母鼠死亡发生。妊娠末期第二周时，G3组大鼠平均体重显著低于G2组（分别为p=0.029与p=0.028）。血液实验室参数分析显示，G1组平均淀粉酶水平最低，G2组平均血红蛋白水平最低且平均血小板计数最高。形态学分析结果表明，母鼠与子代大鼠的肾脏、肝脏等器官均未出现异常改变。G3组中有3只母鼠出现肺部炎症。结论 恩夫韦肽对妊娠、妊娠产物及母鼠脏器功能均无显著不良影响。