CIL:25631

This movie illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of ufd-1 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The movie corresponds to experiments shown in Fig. 1B and Movie 4 of Mouysset et al. Movie is CIL 25631 and original data file is CIL 35126.本影片展示了通过RNA干扰（RNAi）敲低ufd-1基因的秀丽隐杆线虫（Caenorhabditis elegans）胚胎从早期分裂直至四细胞阶段的过程。秀丽隐杆线虫受精卵P0的首次胚胎分裂为不对称分裂，会产生前部AB细胞与体积更小的后部P1细胞。两类细胞具有截然不同的发育命运与分裂时序，AB细胞的分裂时间较P1细胞早约2分钟。通过延时微分干涉差（DIC）显微镜成像观察发现，下调cdc-48、ufd-1与npl-4基因会使P1细胞相较于AB细胞出现更长的分裂延迟，进而延长三细胞阶段的持续时长。本影片对应Mouysset等人研究中图1B及影片4所展示的实验，其编号为CIL 25631，原始数据文件编号为CIL 35126。