Catalan Surveillance Network of SARS-CoV-2 in Sewage

The Catalan Surveillance Network of SARS-CoV-2 in Sewage started in July 2020 to monitor 56 wastewater treatment plants (WWTPs) all over the country representing the sewage from the 80% of the total population. The sampling plan consist in a weekly collection of 24 hours-composed-flow proportional water sample from the entrance of each WWTPs. The raw sewage samples are send to references laboratories for analysis within 24h after collection. Samples are concentrated, the RNA extracted and finally amplyfied using two target genes (N1/N2 or N1/IP4). Here, we periodically release the dataset of the results obtained for each WWTP analysed with sampling date, gene copies per litre for each viral target, the daily flow from the WWTP, rain events registered and extra comments.加泰罗尼亚新型冠状病毒（SARS-CoV-2）污水监测网络于2020年7月启动，旨在对全国范围内的56座污水处理厂（WWTPs）开展监测，这些设施覆盖了全国80%总人口的污水排放。采样方案为每周从各污水处理厂的进水口采集24小时混合比例流量水样。采集后的原污水样本需在24小时内送往参考实验室进行分析。样本将依次经过浓缩、RNA提取步骤，最终使用两种靶标基因（N1/N2或N1/IP4）完成扩增。本研究定期发布经各污水处理厂分析所得的结果数据集，包含采样日期、每升水样中各病毒靶标的基因拷贝数、污水处理厂日流量、记录的降雨事件以及额外备注。