Effect of Ad5-porcine interferon-α pre-treatment in Ebola virus induced gene expression in pigs

Microarray analysis was done to compare the response of EBOV-infected pigs that were pre-treated with Ad5-porIFNα or PBS. At 3 days post EBOV infection, pigs that had been pre-treated with Ad5-porIFNα displayed upregulation of many genes related to the immune response in contrast to pigs treated with PBS. The largest category of induced genes included cytokines and chemokines that affect T-cell differentiation (IL27) and T-cell recruitment (CCL5, CCL23, CCL24, CXCL9, CXCL11) To investigate any modulatory effects of the Ad5-porIFNα pre-treatment on the course of EBOV-induced pathogenesis, we harvested PBMCs from each pig at 3 and 5 or 6 DPI, and the BALF pellet of all animals at the time of necropsy. We then extracted RNA from these samples and performed microarray analysis