7AC_Luciferase assay_lin-28 mutant alleles

Luciferase assay data used to quantify the duration of larval stages of animals of the indicated genotypes. The file contains three sheets: Raw data: Contains the raw values measured by the luminometer (Berthold Technologies, Centro XS3 LB 960). Luminescence was measured every 10 min for 0.5 seconds. The luminometer was placed in a temperature-controlled incubator set to 18.5°C, which translated to an effective temperature of 22.5°C experienced by the animals inside the luminometer. Plate design: Position of animals of indicated genotypes in the 384-well luminometer plate. Annotated data: List of animals of indicated genotypes with the times of hatch, molt entry and exit and calculated durations of molt, intermolts and larval stages in hours. Animals excluded from the analysis are indicated by "0" in the "is_valid" column. wild type HW1993 xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV lin-28(0) HW2706 lin-28(xe192) I; xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV lin-28(n719) HW2790 lin-28(n719) I; xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV本数据集为荧光素酶检测（Luciferase assay）数据，用于定量指定基因型动物的幼虫阶段时长。该文件包含三个工作表： 原始数据（Raw data）：包含化学发光检测仪（luminometer，Berthold Technologies, Centro XS3 LB 960）测得的原始数值。每10分钟检测一次发光信号，单次检测时长为0.5秒。检测仪被置于设定为18.5°C的控温培养箱内，此时仪器内的动物实际感受到的有效温度为22.5°C。 板布局（Plate design）：指定基因型动物在384孔化学发光检测板中的位置信息。 注释后数据（Annotated data）：包含指定基因型动物的列表，以及各动物的孵化时间、蜕皮启动与退出时间，并计算得到了以小时为单位的蜕皮期、蜕皮间期与幼虫阶段时长。分析中被排除的动物会在“is_valid”列中以“0”标记。 野生型（wild type）：品系编号HW1993，基因型为xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV lin-28(0)：品系编号HW2706，基因型为lin-28(xe192) I; xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV lin-28(n719)：品系编号HW2790，基因型为lin-28(n719) I; xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV