Additional file 1: of MAP3K7 is recurrently deleted in pediatric T-lymphoblastic leukemia and affects cell proliferation independently of NF-ÎşB

Supplemental Methods: Details an patient data, AAV vector production, shRNA vector design. Table S1. Primers used for qRT-PCR. Figure S1. Transduction of T-ALL with anti-MAP3K7 shRNA leads to an efficient knockdown. Figure S2. Plasmid map of pscAAV-CMV-GFP-U6-sh construct with anti-MAP3K7 shRNA. Figure S3. Transduction of T-ALL with anti-MAP3K7 shRNA induces apoptosis. (ZIP 3146 kb)补充方法：详述了患者数据、腺相关病毒（AAV）载体制备以及短发夹RNA（shRNA）载体设计。表S1：定量实时聚合酶链反应（qRT-PCR）所用引物。图S1：采用抗MAP3K7短发夹RNA（shRNA）转导T细胞急性淋巴细胞白血病（T-ALL）可实现高效基因敲低。图S2：携带抗MAP3K7短发夹RNA（shRNA）的pscAAV-CMV-GFP-U6-sh重组质粒图谱。图S3：采用抗MAP3K7短发夹RNA（shRNA）转导T细胞急性淋巴细胞白血病（T-ALL）可诱导细胞凋亡。（压缩包大小：3146 kb）