Prolidase metal content.

Mn(II) and Zn(II) content was analyzed by ICP-MS after 48 h dialysis against metal free buffer based on their presence in the enzyme active site as previously demonstrated [4].如先前文献[4]所证实，针对存在于酶活性位点的二价锰离子（Mn(II)）与二价锌离子（Zn(II)），本研究在与无金属缓冲液透析48小时后，采用电感耦合等离子体质谱法（ICP-MS）对其含量进行了分析。