RNA-seq Data from "Mitogen-Activated Protein Kinase–Dependent M2 Macrophage Polarization Mediates the Hepatoprotective Effect of Wenyang Huazhuo Tuihuang Formula in Acute-on-Chronic Liver Failure"

This dataset pertains to the RNA-seq component of the article “Mitogen-Activated Protein Kinase–Dependent M2 Macrophage Polarization Mediates the Hepatoprotective Effect of Wenyang Huazhuo Tuihuang Formula in Acute-on-Chronic Liver Failure”. In this study, transcriptome sequencing of liver tissues was performed on ACLF model mice and mice treated with WYHZH (comprising Aconitum carmichaelii Debeaux [Baifu tablets], Zingiber officinale Roscoe [dried ginger], Artemisia capillaris Thunb. [Yin Chen], Rheum palmatum L. [raw rhubarb], Paeonia lactiflora Pall. [Chishao], Reynoutria japonica Houtt. [Polygonum cuspidatum], Panax ginseng C.A.Mey. [ginseng], Atractylodes macrocephala Koidz. [atractylodes], and Glycyrrhiza uralensis Fisch. [licorice]) using the Illumina NovaSeq 6000 platform. Through differential expression gene screening and GO/KEGG enrichment analysis, this dataset presents the altered expression of MAPK signaling pathway-related targets following herbal formula intervention.本数据集关联于论文《丝裂原活化蛋白激酶依赖性M2巨噬细胞极化介导温阳化浊退黄方对慢加急性肝衰竭的保肝作用》（原英文标题："Mitogen-Activated Protein Kinase–Dependent M2 Macrophage Polarization Mediates the Hepatoprotective Effect of Wenyang Huazhuo Tuihuang Formula in Acute-on-Chronic Liver Failure"）的RNA测序（RNA-seq）部分。本研究使用Illumina NovaSeq 6000测序平台，对慢加急性肝衰竭（Acute-on-Chronic Liver Failure, ACLF）模型小鼠及经温阳化浊退黄方（简称WYHZH）干预的小鼠开展肝组织转录组测序；该复方的组方成分包括：附子（Aconitum carmichaelii Debeaux，白附片）、干姜（Zingiber officinale Roscoe）、茵陈（Artemisia capillaris Thunb.）、生大黄（Rheum palmatum L.）、赤芍（Paeonia lactiflora Pall.）、虎杖（Reynoutria japonica Houtt.，旧学名为Polygonum cuspidatum）、人参（Panax ginseng C.A.Mey.）、白术（Atractylodes macrocephala Koidz.）及甘草（Glycyrrhiza uralensis Fisch.）。后续通过差异表达基因筛选与基因本体（Gene Ontology, GO）/京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）富集分析，本数据集展示了该中药复方干预后丝裂原活化蛋白激酶（Mitogen-Activated Protein Kinase, MAPK）信号通路相关靶点的表达变化情况。