Microbiota Study Crohn's Disease AG Chang

We have investigated a Crohn’s disease (CD) cohort by our multi-parameter microbiota flow cytometry approach to characterise the microbiota on single-cell level for attributes of the disease. The microbiota is isolated from stool samples and stained according to the published protocol for (a) host immunoglobulins IgA1, IgA2, IgM, IgG and (b) agglutinin binding to mannose, galactose or N-Acetyl-glucosamine surface sugar moieties. For all samples we also determined the microbiome composition by 16S rRNA (V3-V4) sequencing on the illumina MiSeq platform. We provide the raw .fcs and FASTQ files of 64 CD patients distributed into two cohorts (55) individuals in cohort 1, 19 individuals in cohort 2). Cohort 2 has two samples for two timepoints. For comparison we additionally analysed 55 healthy donors. All .fcs files were generated on BD Influx®. The metadata is collected in the provided meta.csv. The staining parameters are summarized in provided panel.csv.本研究采用多参数微生物群流式细胞术方法，针对克罗恩病（Crohn’s disease, CD）队列开展研究，旨在从单细胞水平解析与该疾病相关的微生物群特征。研究所涉及的微生物群均从粪便样本中分离获得，并按照已发表的实验方案完成两类染色：（a）宿主免疫球蛋白IgA1、IgA2、IgM、IgG；（b）凝集素与甘露糖、半乳糖或N-乙酰葡糖胺表面糖基的结合反应。针对所有样本，本研究同时采用Illumina MiSeq平台完成16S rRNA（V3-V4区）测序，以解析微生物组组成。本研究公开了64名CD患者的原始.fcs文件与FASTQ文件，所有样本分为两个队列：队列1包含55名个体，队列2包含19名个体。队列2包含对应两个时间点的2份样本。 为开展对照分析，本研究同时纳入55名健康供体样本进行检测。 所有.fcs文件均通过BD Influx®流式细胞仪生成。 样本元数据已整理至提供的meta.csv文件中。染色实验参数已汇总至提供的panel.csv文件中。