Infection and immune status of RT-SHIV-challenged macaques.

ˆCD4 counts taken on day 84 for DE38, day 189 for FI76, FI78, FI70, FI71, FI73, and FI75, and day 196 for FI86 and FI87.$Co-cultures of PBMCs with 174×CEM cells were scored as positive by microscopic examination of syncytia formation and in some instances verified by PCR for SIV gag.&DE38 had two timepoints of positive IFNγ responses at weeks 8 and 10 post challenge.*GF14 - average 715 copies/ml on day 42 post challenge, but the animal remained below the level of detection at all other time points.%500 µM of MIV-150 in MC administered once 30 min prior to challenge (1X) or 24 h before, 30 min before, and 30 min after virus challenge (3X).@These animals exhibited low-level viremia but ultimately controlled infection to undetectable levels even after CD8 depletion.Ab positivity was defined as having at least two positive OD values above baseline at 4 weeks post challenge and IFNγ positivity was defined as having at least 50 SIV-specific IFNγ SFCs per 106 PBMCs on more than one time point post challenge.ND, not determined.ˆ 针对DE38的CD4计数检测于第84天完成，针对FI76、FI78、FI70、FI71、FI73、FI75的CD4计数检测于第189天完成，针对FI86和FI87的CD4计数检测于第196天完成。$ 外周血单个核细胞（PBMC）与174×CEM细胞的共培养物通过显微镜观察合胞体形成情况判定为阳性，部分样本经猴免疫缺陷病毒（SIV）gag基因的聚合酶链式反应（PCR）验证。& DE38在攻毒后第8周和第10周出现两次阳性干扰素γ（IFNγ）应答。* GF14在攻毒后第42天的平均病毒载量为715拷贝/毫升，但该受试动物在其余所有时间点的病毒载量均低于检测下限。% 以500 µM浓度的MIV-150溶于MC中，分别采用两种给药方案：于病毒攻毒前30分钟单次给药（1次给药组），或于病毒攻毒前24小时、攻毒前30分钟以及攻毒后30分钟给药（3次给药组）。@ 受试动物出现低水平病毒血症，但最终即使在CD8细胞耗竭后，仍能将感染控制至检测不到的水平。抗体阳性定义为攻毒后4周至少存在两个高于基线的阳性光密度（OD）值；干扰素γ阳性定义为攻毒后多个时间点中，每10⁶个外周血单个核细胞内至少含有50个SIV特异性IFNγ斑点形成细胞（SFCs）。ND，即未测定（not determined）。