蛋白质表达模式决定了EPSC小鼠类囊胚的形成潜力

植入前胚胎发育是一个由母系遗传和新合成的蛋白质组织的精确调控过程。最近，一些研究报告称，胚泡样结构，称为胚泡，可以由小鼠胚胎干细胞或扩展多能干细胞产生。在本研究中，为了探索蛋白质及其PTMs在小鼠EPS母细胞中的动态表达特征，我们通过基于TMT的定量质谱（MS）策略揭示了EPS母细胞的蛋白质表达谱和代谢产物特征。此外，鉴定了蛋白质磷酸化位点，以显示与小鼠早期胚胎相比，卵裂球中的磷酸化蛋白质组学分析。最重要的是，我们的研究揭示了在植入前发育过程中，与小鼠胚胎相比，EPS卵裂球的蛋白质表达谱，并表明葡萄糖代谢是卵裂球形成的关键。Preimplantation embryonic development is a precisely regulated process orchestrated by maternally inherited and newly synthesized proteins. Recently, multiple studies have reported that blastocyst-like structures, termed blastoids, can be generated from mouse embryonic stem cells or expanded pluripotent stem cells (EPS). In this study, to investigate the dynamic expression profiles of proteins and their post-translational modifications (PTMs) in mouse EPS-derived maternal cells, we uncovered the proteomic profiles and metabolomic characteristics of EPS maternal cells using a TMT-based quantitative mass spectrometry (MS) approach. Additionally, we identified protein phosphorylation sites and conducted phosphoproteomic analysis of blastomeres for comparison with those in mouse early embryos. Most importantly, our study revealed the proteomic expression patterns of EPS blastomeres relative to mouse embryos during preimplantation development, and demonstrated that glucose metabolism is a key determinant for blastomere formation.

搜集汇总

背景与挑战

背景概述

该数据集聚焦于小鼠扩展多能干细胞（EPSCs）来源的类囊胚的蛋白质表达谱研究，通过基于TMT的定量质谱技术揭示了蛋白质及其翻译后修饰的动态特征，并与早期胚胎进行了比较分析。研究发现葡萄糖代谢在类囊胚形成中起关键作用，数据集包含相关的图像数据（如胚胎显微图像），为理解胚胎发育的分子机制提供了重要资源。