The effect of freeze-drying and storage on lactobacilli strains from the gastrointestinal tract of wild boars using different cryoprotectants

Probiotics play an important role in maintaining gastrointestinal comfort and health in piglets. For this reason, lactobacilli isolated from the gastrointestinal tract of domestic pigs and wild boars are evaluated for probiotic properties with the aim of developing probiotics for animal husbandry. Resistance to technological processing is a key requirement for probiotic strains, for which reason the viability of Limosilactobacillus mucosae M65A, Ligilactobacillus salivarius M494A and Lactobacillus amylovorus M624A was assessed after freeze‑drying and after 120 days of storage.益生菌在维持仔猪胃肠道舒适与健康方面发挥着关键作用。为研发适用于畜牧生产的益生菌制剂，研究人员对从家猪与野猪胃肠道中分离得到的乳杆菌开展益生菌特性评价。耐受工艺加工性能是益生菌菌株的核心必备条件，据此本研究检测了粘膜乳杆菌（Limosilactobacillus mucosae）M65A、唾液乳杆菌（Ligilactobacillus salivarius）M494A和解淀粉乳杆菌（Lactobacillus amylovorus）M624A在冷冻干燥后以及储存120天后的活菌存活率。