Soybean root phenotyping dataset and weather dataset from PPAC (Piney Purdue Agricultural Center), IN

This data repository contains records of root phenotypes which were collected in the Pinney Purdue Agricultural Center (PPAC) (Wanatah, Indiana, USA). Briefly, plants were grown in the two sites within PPAC that differed in soil texture, sandy loam and clay loam. In each site, there were three replications per genotype. Plants were hand-sown in single-row plots 3 m in length (2.1 m short row with 0.9 m alley) to a depth of 2.5 cm to 3.8 cm. Roots were collected for all genotypes and both sites across three different developmental timepoints, giving rise to 432 samples altogether. Plant samples were randomly selected from each single row plot for root sample collection. One sample per plot was collected at a time to ensure accuracy. We used shovels to excavate samples, taking care to minimize damage to the root systems, and samples were stored in ziploc bags for transportation to the laboratory. Images of roots were collected using an imaging platform made up of a DSLR camera, copy stand, and light box. Trait extraction was carried out in WinRhizo and ImageJ. The following files contain the data with the root phenotypes and weather data for that season. This work was funded by an AgSEED grant at Purdue University.本数据集仓库收录了采自美国印第安纳州瓦纳塔市平尼普渡农业中心（Pinney Purdue Agricultural Center, PPAC）的根系表型记录。简要而言，实验在PPAC内两个土壤质地存在差异的试验点开展，分别为砂壤土与黏壤土。每个试验点中，每个基因型设置3次生物学重复。作物以单行区组形式人工播种，区组总长3米（其中种植带长2.1米，间隔带宽0.9米），播种深度为2.5厘米至3.8厘米。 针对所有基因型与两个试验点，我们在三个不同的发育时期采集根系样本，总计获得432份样品。从每个单行区组中随机选取植株用于根系采样，每次仅采集一个区组的样品以保障实验准确性。我们使用铁锹挖掘样本，尽量减少对根系系统的损伤，采集后的样品置于自封袋中，运输至实验室保存。 根系图像通过由数码单反相机（DSLR）、翻拍架与柔光箱组成的成像平台采集。表型性状提取工作在WinRhizo与ImageJ软件中完成。 以下文件包含了本季的根系表型数据与对应气象数据。 本研究由普渡大学AgSEED项目资助。