Code analysis and supplementary files for "Molecular responses to Marteilia sydneyi infection in the Sydney rock oyster Saccostrea glomerata"
收藏资源简介:
This dataset contains the de novo transcriptome assembly, gene and protein prediction and annotation, R scripts and workflows used to analyse differential gene expression in Sydney rock oysters (Saccostrea glomerata) infected with the parasite Marteilia sydneyi. The code includes differential expression analysis using DESeq2, gene ontology (GO) enrichment analysis, and visualization of results. These analyses were conducted as part of a study investigating the molecular mechanisms underlying oyster immune responses to parasitic infections. The provided scripts are essential for reproducing the results presented in the associated research paper "Molecular responses to Marteilia sydneyi infection in the Sydney rock oyster Saccostrea glomerata" and serve as a resource for researchers conducting transcriptomic studies on marine invertebrates. For additional analysis such as de-novo transcriptome assembly, please see the linked repository URL below under "Repository URL." For sequencing data please see the following link: http://www.ncbi.nlm.nih.gov/bioproject/1173877
本数据集包含感染悉尼马尔太虫(Marteilia sydneyi)的悉尼岩牡蛎(Saccostrea glomerata)的从头转录组组装(de novo transcriptome assembly)、基因与蛋白质预测注释结果,以及用于开展差异基因表达分析的R脚本与分析流程。其中分析代码涵盖基于DESeq2的差异表达分析、基因本体(Gene Ontology,GO)富集分析,以及结果可视化环节。本系列分析为一项探究牡蛎应对寄生虫感染的免疫应答分子机制的研究的组成部分。所提供的脚本可复现相关研究论文《悉尼岩牡蛎(Saccostrea glomerata)感染悉尼马尔太虫后的分子应答》中的实验结果,同时可为从事海洋无脊椎动物转录组学研究的科研人员提供参考资源。 如需开展从头转录组组装等额外分析,请参见下文‘仓库链接’项下的关联代码仓库地址。 相关测序数据请访问以下链接:http://www.ncbi.nlm.nih.gov/bioproject/1173877



