Table 4 in Identification of a UDP-Glucosyltransferase favouring substrate- and regiospecific biosynthesis of flavonoid glucosides in Cyclocarya paliurus

Table 4 Comparison of the regio-selectivity and activity of C. paliurus GT 1 and its mutants.
EnzymeRegio-selectivity of glucosylationDisaccharides % TTotal (%)3′-OH3-OH7-OH% T a% W b% T% W% T% WWild-type821001210061000100T11A78621581776065M14I––––––––A43F56370044396054F45G6959179914163070A46M8199141175830100A48P7511615159102120127T49F8076106510130078V84P242485468918081V88I7415316227102830170M121F215911839156024F147V314810849221027S189D80981210081330100F190A881488924920138M201Y100790000065W283M731601116539013180G284V81451454538046△V309-D320701431014038417168Vector control00000000
a % T represents the percentage of the HPLC peak area for each product relative to the total product peak area. b % W represents the percentage of the HPLC peak area for each product relative to the same product from an identical incubation with an equal amount of wildtype enzyme.