Legume hyperspectral and in-situ biophysical/biochemical dataset collected in the Southern Plains

A field experiment focused on three legumes (soybeans [Glycine max], mothbeans [Vigna aconitifolia], and tepary bean [Phaseolus acutifolius]) was conducted in El Reno, OK over a two year period (2018, 2019). The split-split plot design for the legumes were subject to various row spacing (38cm and 76cm) and irrigation regimes (irrigated and rainfed). Sampling of the plots took place a total of seven times over the two year period. Each of the samplings included an initial triplicate (averaged) hyperspectral readings using a spectroradiometer (350nm to 2500nm; FieldSpec Pro FR: Malvern Panalytical, Westborough, MA, USA), in-situ measurements (canopy cover [collected with the “Canopeo” app where a ratio of plant to ground pixels were calculated], chlorophyll content [collected with Chlorophyll Content Meter-300, Opti-Sciences, Hudson, NH, USA]), and biomass clipping for various laboratory analytics (dry weight, nitrogen/carbon content, crude protein, neutral detergent fiber, acid detergent fiber, in vitro true digestibility). Locations for sampling (n=334) within the 4m x 3m plots were chosen at random.本田间试验于美国俄克拉荷马州埃尔里诺（El Reno, OK）开展，周期为两年（2018年、2019年），供试豆科作物共3种：大豆（Glycine max）、蛾豆（Vigna aconitifolia）与特帕里菜豆（Phaseolus acutifolius）。该试验采用裂裂区试验设计，设置两个处理因子：行距（38cm、76cm）与灌溉制度（灌溉组、雨养组）。试验于两年周期内共开展7次样地采样。每次采样流程如下：首先利用光谱辐射计（spectroradiometer，波长范围350nm~2500nm；FieldSpec Pro FR型，马尔文帕纳科（Malvern Panalytical）公司，美国马萨诸塞州韦斯特伯勒）采集3次重复高光谱读数并取平均值；随后开展原位测定：包括冠层覆盖度（通过"Canopeo"应用程序计算植株与地面像素占比获得）、叶绿素含量（使用叶绿素含量测定仪-300型，Opti-Sciences公司，美国新罕布什尔州哈德逊采集）；最后剪取生物量样本，用于后续多项实验室分析指标测定，包括干重、氮/碳含量、粗蛋白、中性洗涤纤维、酸性洗涤纤维以及体外真实消化率。在4m×3m的样地内，共随机选取334个采样点位。