Gene Expression in Dunaliella tertiolecta +/- Iron

Dunaliella tertiolecta is an extremophilic, green alga from the Chlorophyte lineage. It is found in coastal marine environments around the world. D. tertiolecta can tolerate extremes of heat, light, pH, and salinity. D. tertiolecta is under development for the production biofuels and other bioproducts because it can produce large quantities of neutral lipids, and it can be grown in open raceway ponds using only the inputs of seawater and sunlight. This isolate of D. tertiolecta (UTEX LB 999) was found in Oslofjord, Norway in 1938. This accession includes an RNA-Seq analysis of D. tertiolecta cultures grown in iron-replete (1.5 µM) or iron-deficient (0 µM) media. Precultures of Dunaliella tertiolecta (UTEX LB 999) were inoculated at 4x10^5 cells/mL into triplicate flasks containing media with 1.5 µM or 0 µM added iron and 2 M NaCl. Cultures were grown on shaking platforms (140 rpm) at 24°C under constant light (100 µE m^-2 sec^-1). After two days of growth, total RNA was collected, poly(A)-selected, and subjected to 50 nt single-end sequencing on the Illumina HiSeq platform. Sequenced data was aligned to the genome assembly included with this accession by RNA STAR. Expression was quantified in terms of Fragments per kb of transcript per million reads mapped (FPKMs) by cuffdiff.