TGF-β in a T cell culture supernatant was measured by the 293T-caga-CD32-CD86-caga bioassay (TGF-β activity), by ELISA without acidification (the 25 kDa free TGF-β dimer), and by ELISA after acidification ( “total TGF-β”).

TGF-β in a T cell culture supernatant was measured by the 293T-caga-CD32-CD86-caga bioassay (TGF-β activity), by ELISA without acidification (the 25 kDa free TGF-β dimer), and by ELISA after acidification ( “total TGF-β”).T细胞培养上清液中的转化生长因子β（Transforming Growth Factor-β, TGF-β）可通过三种方式检测：采用293T-caga-CD32-CD86-caga生物分析法检测其活性；采用未酸化处理的酶联免疫吸附测定（Enzyme-Linked Immunosorbent Assay, ELISA）检测25 kDa游离TGF-β二聚体；采用酸化处理后的ELISA检测"总TGF-β"。