Cunninghamia lanceolata transcriptome

The dark-treatment on new sprouts of Chinese fir caused etiolation via blocking the expression ofdark-operative protochlorophyllide reductase (DPOR) and the light-dependent protochlorophyllide reductase (LPOR) encoding genes. We extracted, sequenced and annotated the total RNA from dark- and light-grown sprouts. A total of 70,499 unigenes were found, of which 47,410 were annotated. Among them, 3263 differentially expressed genes were identified between dark- and light-grown samples. Both nuclear and plastid genomic genes were significantly down-regulated in etiolated sprouts, showing a quit different gene expression pattern to the dark-grown cotyledons.