Revealing Missing Human Protein Isoforms Based on Ab Initio Prediction, RNA-seq and Proteomics

In this project, we design a methods to detect novel transcripts integrating ab initio prediction on genomic sequences and filtration with various RNA-seq data. 100 splice sites were selected and RT-PCR on 48 human tissues and cell lines (including a blank negative control) were conducted. The product of the same tissue or cell line were mixed and sequenced with Illumina Mi-seq.