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TNF response in promyelocytic and granulocytic forms of HL60/S4 cells

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下载链接:
https://www.ncbi.nlm.nih.gov/sra/SRP162760
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资源简介:
The human promyelocytic cell line HL60/S4 can be differentiated into a granulocytic form with the addition of all-trans retinoic acid for 4 days. We investigated the conserved and differentiated responses to TNF in the granulocytic and promyelocytic forms of HL60/S4 cells. Overall design: Promyeloytic and granulocytic HL60/S4 cells were treated with 16ng/ml TNF or equivalent volume of vehicle (water) in calcium supplemented growth media for two hours, then RNA was extracted with Trizol LS and the Qiagen Rneasy mini kit.
创建时间:
2019-09-24
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