Motion-corrected calcium image files
收藏资源简介:
Processed images from "Raw image files". For each raw image file, channel 2 (the GCaMP emission channel) was extracted and motion-corrected using the normcorre image registration pipeline (https://github.com/flatironinstitute/NoRMCorre). These files can be loaded into suite2p (https://www.suite2p.org/) for automatic cell segmentation, fluorescence extraction, and spike inference. Full details as to how these files were analyzed are available here: https://github.com/sn-lab/MouseGoggles/tree/main/Publication
本数据集包含「原始图像文件」的处理后图像。针对每一份原始图像文件,我们提取了第2通道——即GCaMP发射通道(GCaMP emission channel)——并使用normcorre图像配准流程(normcorre image registration pipeline,https://github.com/flatironinstitute/NoRMCorre)完成了运动校正。上述处理后的图像文件可加载至suite2p(https://www.suite2p.org/)中,用于自动细胞分割、荧光信号提取与锋电位推断。关于这些文件的完整分析流程细节,可通过以下链接获取:https://github.com/sn-lab/MouseGoggles/tree/main/Publication



