Conservation genomics of the Kuranda Treefrog (Litoria myola) - Filtered SNP data

This dataset contains the filtered neutral SNP data (n=7132, genepop format) produced with DArTseq at Diversity Arrays Technology, Canberra. <br> Adult individuals were sampled throughout the species range between 2000 and 2016, with two main sampling events (2001-2004; 2007-2009). <br> The library preparation consisted of a dougle digest restriction with PstI and SphI. Fragments were amplified with 30 rounds of PCR. No size selection was conducted. Equimolar amounts of the individual libraries were pooled and sequenced on an Illumina HiSeq 2500 (77-bp single end). The resulting raw data was genotyped with DArT's KDCompute, and then filtered in R and excel for Call Rate (&lt; 0.9), MAF (&lt; 0.02), Reproducibility (&lt;0.9). Single calls with a read depth below 10 were silenced. Monomorphic loci were removed. From each pair of siblings only the one with the higher call rate was retained. <br> Additionally, a metadata file containing sex, year and location of sampling is provided. A readme file is included.本数据集包含经筛选的中性单核苷酸多态性（Single Nucleotide Polymorphism, SNP）数据（共7132个位点，格式为genepop格式），由位于堪培拉的多样性阵列技术公司（Diversity Arrays Technology）通过DArTseq技术生成。 研究于2000年至2016年间在该物种的整个分布范围内采集成年个体样本，主要分为两次采样阶段（2001-2004年；2007-2009年）。 文库构建采用PstI与SphI进行双酶切限制性酶切，酶切所得片段通过30轮聚合酶链式反应（Polymerase Chain Reaction, PCR）扩增，且未进行片段大小筛选步骤。将等摩尔浓度的各独立文库混合后，在Illumina HiSeq 2500测序平台上进行77bp单端测序。所得原始数据通过DArT公司的KDCompute平台完成基因分型，随后在R语言与Excel软件中按照以下标准进行筛选：分型成功率（Call Rate）<0.9、最小等位基因频率（Minor Allele Frequency, MAF）<0.02、重复性（Reproducibility）<0.9；测序深度低于10的单次分型结果将被标记为缺失；单态性位点已被移除；每对同胞个体中仅保留分型成功率更高的那一个样本。 此外，本数据集附带一份包含采样个体性别、采样年份与采样地点的元数据文件，同时包含说明文档（readme文件）。