BiCCA: Bi-order multiomics integration of single-cell data

Chimeric antigen receptor (CAR) engineering of NK cells is an active area of research with early-phase clinical studies showing an excellent safety profile with encouraging clinical responses. However, the transcriptional signatures that control the fate of CAR-NK cell after infusion and their association with tumor control remain poorly understood. Here, we performed single-cell RNA sequencing (scRNA-seq) to depict the evolution of various engineered CAR-NK cells Single cell RNA sequencing (scRNA) has revolutionized high-thoughout systems-based analysis of cellular and functional heterogeneity, The goals of this work are to compare transcriptome profiling (RNA-seq). The objective of this study was to investigate the kinetics of function and evolution of adoptively-transferred CAR-NK cells in the context of lymphoma treatment. Of particular interest to us was to determine the impact of IL-15 on the function and persistence of CAR-NK cell and to assess the contribution of IL-15 to NK-mediated anti-tumor activity. To accomplish this, we applied a multi-modal omics approach to characterize pre-infusion NK products and describe the evolution of NK cells post-infusion.