Synthesis of hydrazinylthiazole carboxylates: a mechanistic approach for treatment of diabetes and its complications

The inhibition potential of compounds 3a–n was found to be dose-dependent, as shown in Supplementary Table 2.Compounds 3a–n were tested for their in vitro antiglycation activity using aminoguanidine as reference inhibitor (Supplementary Table 3).The free radical scavenging ability of compounds 3a–n was estimated using the DPPH scavenging model. Ascorbic acid was used as positive control, and percentage antioxidant ability and IC50 values are presented in Supplementary Table 4.

研究发现，化合物3a–n的抑制潜力呈剂量依赖性，如补充表2所示。通过以氨基胍作为参照抑制剂，对化合物3a–n的体外抗糖基化活性进行了测试（见补充表3）。采用DPPH清除模型评估了化合物3a–n的自由基清除能力。以抗坏血酸作为阳性对照，补充表4中展示了抗氧化活性的百分比及IC50值。