Additional file 1: of Swift metabolite changes and leaf shedding are milestones in the acclimation process of grapevine under prolonged water stress

Table S1. Stomatal conductance measurements of 2014 and 2015 presented as mean (N = 4) and standard error. The respective time points are indicated with days from treatment application. Table S2. Fold change (Treatment/Control) of leaf secondary metabolites during the time course of the experiment in 2014 and 2015. Table S3. Fold change (Treatment/Control) of leaf central metabolites during the time course of the experiment in 2014 and 2015. Table S4. Summary of RNA sequencing analysis metrics. The table show the number of filtered reads and aligned reads (filtered) to the 12X grapevine reference genome that are counted. Table S5. Transcript abundance of the all 29,970 genes, reported as Reads Per Kilobase of transcript, per Million mapped reads (RPKM), in leaves of well-watered and water deficit treated grapevines at day 4, 8 (2014) and day 12 (2015). Tables S6–S8. Summary of differentially expressed genes (FDR 1) in grapevine leaves comparing well-watered and water deficit treatments. DESeq2 statistical outputs of day 4, 8 (2014) and day 12 (2015) treatments are listed in Table S6, S7, and S8, respectively.. Annotation of genes were referred from Vitisnet ( https://www.sdstate.edu/agronomy-horticulture-and-plant-science/functional-genomics-bud-endodormancy-induction-grapevines-5 ) (Grimplet et al. 2009). Table S9. List of differentially expressed genes in grapevine leaves that are common and unique leaves in each intersection of the Venn diagram described in Fig. 4b. Table S10. Summary of enriched (FDR < 0.05) plant GO slim categories in grapevine leaves comparing well-watered and water deficit treatments at day 4, 8 (2014) and day 12 (2015). Other statistical outputs of BiNGO (Maere et al. 2005) are reported. Table S11. Summary of cis-regulatory elements (CRE) enriched at a threshold of FDR < 0.01 in promoters of drought-regulated genes at day 4, 8 (2014) and day 12 (2015). For each treatment group, the number of genes containing the CRE, group size, total number of genes in the genome containing the CRE, promoter size, enrichment P-value, FDR, and lists of genes containing the CRE with their CRE position along the gene promoter are reported. (XLSX 3596 kb)表S1：2014年与2015年的气孔导度（stomatal conductance）测量结果，以平均值（N=4）和标准误（standard error）形式呈现，对应时间点以处理实施后的天数标注。表S2：2014年与2015年实验时间进程中，葡萄叶片次生代谢物的倍数变化（处理组/对照组）。表S3：2014年与2015年实验时间进程中，葡萄叶片中心代谢物的倍数变化（处理组/对照组）。表S4：RNA测序（RNA sequencing）分析指标汇总。该表格列出了经过滤的reads数量，以及比对至12X葡萄参考基因组（reference genome）的过滤后比对reads的计数数量。表S5：2014年第4、8天以及2015年第12天，充分供水与水分胁迫处理的葡萄叶片中，全部29970个基因的转录本丰度，以每百万比对reads的转录本每千碱基读数（Reads Per Kilobase of transcript, per Million mapped reads, RPKM）报告。表S6至S8：对比充分供水与水分胁迫处理的葡萄叶片中差异表达基因（differentially expressed genes）的汇总（FDR=1）。2014年第4、8天以及2015年第12天处理组的DESeq2统计输出结果，分别列于表S6、S7和S8。基因注释参考自Vitisnet数据库（https://www.sdstate.edu/agronomy-horticulture-and-plant-science/functional-genomics-bud-endodormancy-induction-grapevines-5）（Grimplet等人，2009）。表S9：葡萄叶片中差异表达基因列表，涵盖图4b韦恩图各交集中的共有基因与特有基因。表S10：2014年第4、8天及2015年第12天，对比充分供水与水分胁迫处理的葡萄叶片中，以FDR < 0.05为阈值富集的植物基因本体slim集（GO slim）类别汇总。同时报告了BiNGO工具（Maere等人，2005）的其他统计输出结果。表S11：2014年第4、8天及2015年第12天，干旱调控基因启动子区域中，以FDR < 0.01为阈值富集的顺式调控元件（cis-regulatory elements, CRE）汇总。针对每个处理组，本表格将报告包含该CRE的基因数、组样本量、基因组中包含该CRE的基因总数、启动子长度、富集P值、错误发现率（FDR），以及包含该CRE的基因列表及其CRE在基因启动子上的位置。本数据集文件格式为XLSX，大小3596 kb。