Table 2 in Vitamin E and reduced glutathione in Prochilodus lineatus (curimba) semen cryopreservation (Characiformes: Prochilodontidae)

Table 2. Means and standard deviations of the rate (%) of semen motility (n = 7) and duration (s) of curimba (P. lineatus) semen after freeze-thaw (cryopreservation) using different antioxidants (n = 6) and using water and bicarbonate as semen activators. ns There were no significant differences in the duration of semen motility between treatments (P>0.05).* There were significant differences between the different activators (P<0.05). VE50: 50 µM, VE100: 100 µM and VE250: 250 µM of vitamin E; RG0.5:0.5 mM, RG1.0:1.0 mM and RG1.5: 1.5 mM of reduced glutathione. TreatmentsRate of semen motility (%)Duration semen motility (s)ActivatorsActivators*WaternsBicarbonatensWaterBicarbonateControl65.71 ± 2.7867.86 ± 2.45164 ± 94b244 ± 70ªVE5070.00 ± 5.8170.71 ± 4.46156 ± 94b227 ± 59ªVE10076.86 ± 4.3065.00 ± 0.42177 ± 75b269 ± 52ªVE25072.14 ± 7.3374.29 ± 6.99176 ± 99b223 ± 52ªRG0.567.86 ± 4.8076.43 ± 6.39147 ± 86b246 ± 55ªRG1.067.14 ± 3.7064.29 ± 0.08156 ± 88b235 ± 64ªRG1.575.71 ± 9.8970.00 ± 3.96166 ± 92b255 ± 82ª表2 采用不同抗氧化剂（n=6）及水、碳酸氢盐作为精子激活剂时，经冻融（低温冷冻保存（cryopreservation））后巨脂鲤（*P. lineatus*）精子活力（semen motility）百分比（n=7）与持续时长（秒）的均值±标准差。 注：ns 表示各处理组间精子活力持续时长无显著差异（*P*>0.05）；* 表示不同激活剂组间存在显著差异（*P*<0.05）。其中：VE50为50 μM维生素E（vitamin E），VE100为100 μM维生素E，VE250为250 μM维生素E；RG0.5为0.5 mM还原型谷胱甘肽（reduced glutathione），RG1.0为1.0 mM还原型谷胱甘肽，RG1.5为1.5 mM还原型谷胱甘肽。 | 处理组 | 精子活力百分比（%） | 精子活力持续时长（s） | 水激活组 | 碳酸氢盐激活组 | | ------ | ------------------ | -------------------- | -------- | -------------- | | 对照组 | 65.71 ± 2.78 | 67.86 ± 2.45 | 164 ± 94^b | 244 ± 70^a | | VE50 | 70.00 ± 5.81 | 70.71 ± 4.46 | 156 ± 94^b | 227 ± 59^a | | VE100 | 76.86 ± 4.30 | 65.00 ± 0.42 | 177 ± 75^b | 269 ± 52^a | | VE250 | 72.14 ± 7.33 | 74.29 ± 6.99 | 176 ± 99^b | 223 ± 52^a | | RG0.5 | 67.86 ± 4.80 | 76.43 ± 6.39 | 147 ± 86^b | 246 ± 55^a | | RG1.0 | 67.14 ± 3.70 | 64.29 ± 0.08 | 156 ± 88^b | 235 ± 64^a | | RG1.5 | 75.71 ± 9.89 | 70.00 ± 3.96 | 166 ± 92^b | 255 ± 82^a |