Metabarcoding to Detect Invasive Fishes data

Data associated with a DNA metabarcoding study of fish (invasive and native) biodiversity in four rivers in Ontario Sub directories 00_sample_map: Map of sampling locations and abundance of larval fishes collected at each site 01_database_build: Reference genetic database and taxonomic information for classification of metabarcoding sequencing reads 02_qiime2_analysis: De-noising and dereplicating DNA metabarcoding data 03_read_correction: Correction for contamination using no-template controls and single fish samples 04_sampling_stats: General summary statistics of sequencing 05_river_richness: Summaries and analyses of species richness across rivers 06_gear_richness: Comparison of gear types on species detected 07_primer_consistency: Comparison of primer target gene on species detected 08_sampling_depth: Comparison of number of samples collected to read depth per sample 09_database_comparison: Comparison of the reference databases for COI and 12S 10_amplicon_distance: Comparison of the genetic distance for COI and 12S metabarcoding primers

本数据集关联一项针对安大略省四条河流中鱼类（入侵种与本土种）生物多样性的DNA宏条形码（DNA metabarcoding）研究。各子目录详情如下：
00_sample_map：采样点位地图及各站点采集的幼鱼丰度数据
01_database_build：用于宏条形码测序读段分类的参考遗传数据库与分类学信息
02_qiime2_analysis：DNA宏条形码数据的去噪与去重复处理
03_read_correction：借助无模板对照与单鱼样本开展污染校正
04_sampling_stats：测序数据的通用汇总统计量
05_river_richness：跨河流的物种丰富度汇总与分析
06_gear_richness：不同采样渔具对物种检出效果的比较
07_primer_consistency：不同引物靶基因对物种检出一致性的比较
08_sampling_depth：采集样本数与单样本测序读深度的相关性对比
09_database_comparison：COI与12S参考数据库的对比分析
10_amplicon_distance：COI与12S宏条形码引物对应的扩增子遗传距离比较