Abdominal angiostrongyliasis can be diagnosed with a immunochromatographic rapid test with recombinant galactin from Angiostrongylus cantonensis

Angiostrongylus costaricensis is the causative agent of abdominal angiostrongyliasis, a zoonotic infection that may produce severe eosinophilic enterocolitis or hepatitis in humans. Parasites are usually not released in stools and serology has an important role in diagnosis. Since cross-reactivity is demonstrated between A. costaricensis and another metastrongylid worm, A. cantonensis, we tested heterologous recombinant galectin as a probe in an immunochromatographic rapid diagnostic test (ICT-RDT) for detection of anti-A. costaricensis antibodies. Almost all (11/12) positive control sera from A. costaricensis infected patients were positive at ICT RDT. These are preliminary indications that r-galectin ICT-RDT is useful for diagnosing A. costaricensis infection.哥斯达黎加管圆线虫（Angiostrongylus costaricensis）是腹部管圆线虫病的病原体，该人畜共患感染可导致人类出现严重嗜酸性粒细胞性小肠结肠炎或肝炎。该寄生虫通常不会随粪便排出体外，因此血清学检测在其诊断中具有重要价值。鉴于已有研究证实哥斯达黎加管圆线虫与另一种后圆线虫科蠕虫——广州管圆线虫（Angiostrongylus cantonensis）之间存在交叉反应，我们将异源重组半乳糖凝集素作为探针，应用于免疫层析快速诊断试验（ICT-RDT）以检测抗哥斯达黎加管圆线虫抗体。在来自哥斯达黎加管圆线虫感染患者的阳性对照血清中，几乎全部（11/12）在ICT-RDT中呈阳性反应。上述结果初步表明，重组半乳糖凝集素免疫层析快速诊断试验可用于哥斯达黎加管圆线虫感染的临床诊断。